| Microorganism name |
Trichoderma reesei
|
| Target proteins |
Chymosin B (Uusitalo et al., 1991)
|
| Wild-type or GMO |
GMO (Uusitalo et al., 1991)
|
| Production mode (intracellular/extracellular) |
Extracellular (Uusitalo et al., 1991)
|
| Protein yield (g/L or g/g?) |
NA |
| Temperature used in study |
NA |
| pH used in study |
NA |
| C & N source |
Whey extract, peptone, (NH4)2SO4 (Uusitalo et al., 1991)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
Danisco recieved FDA GRAS approval for the production of chymosin B in T. reesei. Not allowed in Canada
|
| Companies |
Danisco |
| Publications/references |
-
Uusitalo, J. M., Nevalainen, K. H., Harkki, A. M., Knowles, J. K., & Penttilä, M. E. (1991). Enzyme production by recombinant Trichoderma reesei strains. Journal of Biotechnology, 17(1), 35–49. https://doi.org/10.1016/0168-1656(91)90025-q
-
Nicolas Hardy, Frédéric Augier, Alvin W. Nienow, Catherine Béal, Fadhel Ben Chaabane. Scaleup agitation criteria for Trichoderma reesei fermentation. Chemical Engineering Science, 2017, 172, pp.158-168. ff10.1016/j.ces.2017.06.034ff. ffhal-01544606f
-
Thiviya, P., Gamage, A., Kapilan, R., Merah, O., & Madhujith, T. (2022). Single Cell Protein Production Using Different Fruit Waste: A Review. Separations, 9(7), 178. https://doi.org/10.3390/separations9070178
-
Alcantara, J., & Mondala, A. (2021). Optimization of Slurry Fermentation for Succinic Acid Production by Fungal Co-culture. DOAJ (DOAJ: Directory of Open Access Journals). https://doi.org/10.3303/cet2186255
-
He, J., Wu, A., Chen, D., Yu, B., Mao, X., Zheng, P., Yu, J., & Tian, G. (2014). Cost-effective lignocellulolytic enzyme production by Trichoderma reesei on a cane molasses medium. Biotechnology for Biofuels, 7(1), 43. https://doi.org/10.1186/1754-6834-7-43
-
Garvey, M. (2022). Non-Mammalian Eukaryotic Expression Systems Yeast and Fungi in the Production of Biologics. Journal of Fungi, 8(11), 1179. https://doi.org/10.3390/jof8111179
-
Nakari-Setala, T., Penttila, M., Bailey, M., Tenkanen, M., & Individual. (2001, January). CA2382468A1 - A method for decreasing foam formation during cultivation of trichoderma - Google Patents. Retrieved August 22, 2025, from https://patents.google.com/patent/CA2382468A1/en
-
Chai, S., Zhu, Z., Tian, E., Xiao, M., Wang, Y., Zou, G., & Zhou, Z. (2022). Building a Versatile Protein Production Platform Using Engineered Trichoderma reesei. ACS Synthetic Biology, 11(1), 486–496. https://doi.org/10.1021/acssynbio.1c00570
|